HDAC3 ChIP sequencing of L4-L6 Dorsal Root Ganglia upon spinal or sciatic axonal injury

Purpose: A) compare the response to an axonal injury in the peripheral vs central nervous system B) clarify the role of HDAC3 in axonal regeneration Results: we found that after peripheral axonal injury there is a higher number of differentially expressed genes regulated by HDAC3 with respect to central injury with a number of genes associated with regenerative pathways Method:HDAC3 ChIP-seq from sciatic L4-L6 DRG tissue 24 hours after the following treatments: a) spinal cord dorsal column injury b) laminectomy c) sciatic nerve injury d) sham. Pool of sciatic DRGs from 20 mice were used for each sample. DRG were collected and flash frozen.