Identification of 14-3-3ε Substrates from Embryonic Murine Brain

Mice deficient in 14-3-3ε exhibit abnormal neuronal migration and die perinatally. We report here the
first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from
embryonic murine brain 163 14-3-3ε interacting proteins and 85 phosphorylation sites on these proteins.
Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its
interaction with 14-3-3ε and for maintaining USP8 in the cytosol.
Keywords: phosphoproteomics • 14-3-3 • epsilon • brain development • kinase • phosphorylation • mass
spectrometry • deubiquitination