Mycn regulates intestinal development through ribosomal biogenesis in a zebrafish model of Feingold syndrome 1 [single-cell RNA-seq]

Purpose: To systematically investigate the phenotypes resulting from Mycn loss-of-function at a higher resolution. Methods: Approximately 30 mycn mutant or WT zebrafish embryos at 72 hpf (hours post fertilization) were harvested . Libraries were prepared using Chromium Controller and Chromium Single Cell 3'Library & Gel Bead Kit v3 (10x Genomics, PN-1000074) according to the manufacturer's protocol for 10000 cells recovery. Results: A total of 25,886 single-cell transcriptomes were collected after stringent quality control measures. Conclusions: 27 Cell types were identified by intergrating two datasets and 9 subclusters were obtained by reclustering intestinal cells. Overall design: wild-type or mycn zebrafish embryos at 72 hpf were harvested for scRNA-seq.