Bglu3 congenic mice - gene expression in liver

A congenic line was constructed by introgressing a C3H chromosome 1 region harboring Bglu3 into C57BL/6 apoE-/- background. RNA was extracted from liver using a QIAGEN kit . Total RNA was pooled in an equal amount from 3 mice for each group. Standard Affymetrix procedures were performed using 8ug of total RNA. Microarrays were used to detect gene expression in the liver of Bglu3 congenic mice and C57BL/6 apoE-deficient mouse strains fed a western diet. 2 groups of mice were studied: C57BL/6 apoE-/- control mice and Bglu3 congenic mice. After fed a Western diet for 12 weeks, liver was harvested and total RNA prepared.本研究构建了Bglu3同源导入系（congenic line）：将携带Bglu3基因位点的C3H小鼠1号染色体区域，同源导入至C57BL/6背景的载脂蛋白E基因敲除（apoE-/-）小鼠基因组中。本研究设置两组受试小鼠：C57BL/6 apoE-/-对照小鼠与Bglu3同源导入系小鼠。两组小鼠均饲喂西式饲料12周，饲喂结束后采集肝脏组织并制备总RNA。实验操作如下：使用QIAGEN试剂盒从肝脏组织中提取总RNA，每组取3只小鼠的总RNA按等量混合；取8微克总RNA，按照标准Affymetrix流程开展实验，采用基因芯片（Microarrays）检测两组小鼠的肝脏基因表达水平。