Chromatin remodeler CHD8 in CNS myelination and remyelination

Mutations in CHD8, an ATP-dependent chromodomain-helicase-DNA-binding protein 8, are known to cause a set of Autism Spectrum Disorders (ASD). We found that CHD8 is highly expressed in myelinating oligodendrocytes (OL) and their progenitors in the developing central nervous system (CNS).We performed CHD8 chIP-seq analysis in cultured oligodendrocytes.CHD8作为一种ATP依赖的染色质域-解旋酶-DNA结合蛋白8，其突变已被证实可引发一系列孤独症谱系障碍（Autism Spectrum Disorders, ASD）。本研究发现，CHD8在发育中的中枢神经系统（central nervous system, CNS）的髓鞘化少突胶质细胞（myelinating oligodendrocytes, OL）及其前体细胞中呈高表达。我们在培养的少突胶质细胞中开展了CHD8的染色质免疫沉淀测序（chromatin immunoprecipitation sequencing, ChIP-seq）分析。