Comparison of syncytia number and nuclei count after syndecan-1 overexpression in each CHO cell type.

Average number of syncytial cells, as well as, the average number of nuclei per syncytia was counted in CHO-K1 and CHO-745 cells after overexpressing syndecan-1 on target cells or effector cells. Positive controls are target and effector cells expressing normal levels of syndecan-1. Syncytial cells were counted 72 h post mixing. Syncytia were classified as any red cell having two or more nuclei. Number of syncytia was normalized to the number of syncytia detected in the negative control wells where the effector cell population lacks gB. The average is based on results from two independent experiment performed in duplicate (mean ± 1SD).