MicroRNA expression profiles do not distinguish between primary and recurrent well-differentiated liposarcoma

Well-differentiated liposarcoma (WDLPS) recurs in approximately one-third of the patients. The molecular relationship between primary tumor and recurrent tumors is barely studied, but is important to reveal potential drivers of recurrence. Here we investigated the biology of recurrent WDLPS using 27 paired primary and recurrent WDLPS samples. MicroRNA expression profiles were determined using TaqMan® Low Density Array (TLDA)-cards. In the supervised clustering analyses, no clear clustering separating the primary from the recurrent tumors, based on differentially expressed microRNAs was observed. The clustering was also not based on tumor localization, time to recurrence, age or status of the resection margins. Subgroup analysis for tumors localized in the extremity or retroperitoneum also did not yield a clear distinction between primary and recurrent WDLPS. In conclusion, microRNA expression profiles do not distinguish between primary and recurrent WDLPS and no putative common drivers for recurrence could be identified. In order to identify common alterations in miRNA expression levels that may drive well-differentiated liposarcoma (WDLPS) recurrence, the miRNA expression profiles of 27 paired tumor samples were determined. Each sample pair consisting of a primary tumor and matching first recurrent WDLPS from patients that were treated with surgery only.