An in vivo massively parallel platform for deciphering tissue-specific regulatory function [RNA]
收藏NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP418351
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资源简介：
Massively parallel reporter assays (MPRAs) have the potential to link differences in genome sequence, including genetic variants, to tissue-specific regulatory function. Whole-animal MPRA (WhAMPRA), where systemic intravenous AAV effectively transduces the plasmid MPRA library to mouse tissues. This technology simultaneously determines the transcriptional functions of hundreds of enhancers in vivo across multiple tissues Overall design: A plasmid libratry with 642 synthetic enhancers and variants each with 20 DNA barcodes was transduced into C57BL6/J mice or transfected into HMC3 cells. DNA barcode transcripts from cDNA were then sequenced.
创建时间：
2025-05-01
