Genotyping results of the FabaPanel for: Genetic erosion within the Fabada dry bean market class revealed by high-throughput genotyping

This dataset contains the results of the genotyping of a set of 180 homozygous bean lines. 
The dataset includes a VCF file with the genotypic data obtained from 'genotyping by sequencing'. The data set was used to perform the study 'Genetic erosion within the Fabada dry bean market class revealed by high-throughput genotyping.'
ABSTRACT 
The Fabada market class within the dry beans has a well-differentiated seed phenotype with very large white seeds. This work investigated the genetic diversity maintained in the seed collections within this market class and possible genetic erosion over the last 30 years. A panel with 100 accessions maintained in seed collections for 30 years, 57 accessions collected from farmers in 2021, six cultivars developed in SERIDA, and 16 reference cultivars were gathered and genotyped, revealing 108,585 SNPs using Genotyping-by-Sequencing. Filtering based on genotypic and phenotypic data was carried out in a staggered fashion to investigate the genetic diversi..., DNA isolation and genotyping
Genomic DNA was isolated from young leaf samples using the SILEX method (Vilanova et al., 2020), and DNA quality was checked in agarose gels. The Genotyping-by-Sequencing (GBS) method was carried out in accordance with Elshire et al. (2011) and optimized by Schröder et al. (2016) using the TaqαI and MseI restriction enzymes. Library construction was performed following the protocol of Poland et al. (2012) with modifications in the adaptors for ligation. In total, 20 barcoded samples were pooled for PCR amplification. Sequencing was performed in the Illumina platform by Macrogen Inc. (Seoul, Republic of Korea). SNP calling was carried out by AllGenetics&Biology SL (www.allgenetics.eu) using the reference genome of Phaseolus vulgaris (Pvulgaris_442_v2.0) obtained from the JGI Data Portal (https://phytozome-next.jgi.doe.gov/info/Pvulgaris_v2_1). , The data can be visualized in Tassel v5, for example.