Dynamic Exosome-Protein and miRNA Crosstalk Modulates Sperm Survival and Motility During Ovarian Storage in Sebastes schlegelii

This study investigated the impact of exosomes, released by ovarian cells in Sebastes schlegelii, on the vitality of stored sperm from a novel perspective. Comprehensive identification and analysis of the contents of ovarian fluid exosomes during the early and late stages of sperm storage (Sst_E and Sst_L) were performed using proteomics and small RNA sequencing. This study aimed to elucidate the interactive effects between exosomes, the ovary, and sperm under two distinct sperm storage states.