Carbon/nitrogen metabolism in responses to phosphate deficiency as revealed by comparative transcriptome profiling in nodules of the model cultivar Williams 82 (soybean)

To understand the responses of plants to environmental stresses will help mitigate the problems via creating stress-tolerant crop.Whole genome DNA microarrays were used to compare the nodule transcriptome profiling of soybean ‘Williams 82’ grown in vermiculite and subjected to low (0.1 mM; LP) or adequate (2.0 mM; HP) Pi levels. Plants were inoculated with two Bradyrhizobium diazoefficiens strains (USDA110 vs. CB1809), which exhibited contrasting growth and nodulation patterns under LP conditions, with the former model strain displaying a lower Pi-stress acclimatization in combination with the model Williams 82. Affymetrix’s whole Soybean Gene Expression Microarray (66K) was used. Microarrays were used to compare the nodule transcriptomes of the model soybean ‘Williams 82’ plants grown in vermiculite and subjected to low (0.1 mM) or sufficient (2.0 mM) inorganic phosphate levels Plants were inoculated with two Bradyrhizobium diazoefficiens strains (USDA110 vs. CB1809) and exposed and to low (0.1 mM) or sufficient (2.0 mM) Pi levels. Nodule samples were collected at 35 day after sowing for RNA extraction and hybridization on Affymetrix microarrays.