Fgf8 E18.5 hypomorph versus control mouse fetal lungs

We set out to look for genome wide gene expression changes due to deficiency of Fgf8 in the lung. RNA from 3 lungs from each genotype (mutant and littermate wild type control) are pooled for each cDNA synthesis and hybridized to individual array for a quadruple biologic repeat of the experiment本研究旨在探究肺组织中成纤维细胞生长因子8（Fgf8）缺失所引发的全基因组基因表达变化。本实验中，我们将每种基因型（突变体与同窝野生型对照）的3份肺组织提取的RNA分别混合，用于互补DNA（cDNA）合成；随后将每份混合样本分别与独立的基因芯片进行杂交，以此完成该实验的四次生物学重复。