TGF-ß treatment of human melanoma cell lines

To analyse whether the activation of TGF-ß signalling (recombinant hTGF-ß, 5 ng/mL; PeproTech) is able to invoke a full phenotype- switch, we performed RNA sequencing of melanoma cultures from two independent melanoma cell lines at two different time point after TGF-ß1 (5 ng/ml, preprotech). Overall design: Control and treated replicates were processed using using TRIsure lysis buffer (BioLine, BIO-38032) according to the directives of the manufacturer. RNA quality was assessed using the 2100 Bioanalyzer (Agilent) prior to expression profiling.