Novel UL23 and UL30 substitutions in HSV1 and HSV2 viruses related to polymorphism or drug resistance

The emergence of resistant herpes virus infections is a delicate situation to manage in immunocompromised patients, especially in those with hematopoietic stem cells transplant. Reactive diagnostic is thus necessary but data about Herpes simplex virus (HSV) polymorphism and acyclovir (ACV) and foscarnet (FOS) resistance mutations is not exhaustive and can represent a brake for an accurate diagnosis. Currently, next generation sequencing (NGS) is more and more used and has been reported as an added value compared to Sanger sequencing, particularly for the detection of minority subpopulations (Mercier-Darty et al., 2019). Herein, we report not previously described UL23 and UL30 substitutions for both HSV1 and HSV2, identified in immunocompromised patients from hematology departments during the last 6-year of HSV resistance surveillance at a University Hospital.