qRT-PCR analysis of gene expression in VOT-E36 two days after differentiation in vitro.

The table includes the 26 genes from the final cluster to gata3 and 9 from the IGF and Akt/PI3K signaling pathways for which we detected a significant change in expression following knock-down of gata3. *Cdkn1b (p27kip1) was not in the final cluster to gata3 but is included here as a potential target of PKB/Akt. The correlation between expression levels from the arrays (column 2) and from Taqman analysis (column 3) for all 61 genes assessed by qPCR was highly significant and is shown in figure 3. Following knock-down of gata3, the expression levels of 9 of the 26 genes within the original gata3 cluster changed significantly (7 down, 2 up, 10 unchanged, 7 undetected/no probe). Significant changes in expression were also observed for 8 genes outside the gata3 cluster but in the IGF signaling pathway. These included igf and igf2 but not igf1r. The threshold for change was set at a ����CT value of 0.5 following subtraction of the variance.