Molecular analysis of transgenic events.
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(A) Binary vector pSB130-AtMAP18. (B) PCR analysis of T0 transgenic lines. PCR was carried out using primers corresponding to the AtMAP18 sequence (top) and hpt sequence (bottom). M: DNA markers, CK−: wild-type (hybrid 08×178), CK+: plasmid pSB130-AtMAP18. (C) PCR analysis of T1 transgenic lines. PCR was carried out using primers corresponding to the AtMAP18 sequence (top) and hpt sequence (bottom). (D) Dot blot analysis of transgenic lines. Blot 1–5: FA7; blot 6–11: FA8; blot 12–18: FA9; blot 19–28: FA12. FA1–FA13: transgenic lines. (E) RT-PCR analysis of the AtMAP18 transcript in the transgenic lines. (F) Western blot analysis. CK+: AtMAP18 protein in Escherichia coli.
创建时间:
2016-02-23



