Distribution of X-ray induced γH2AX foci in relation to HP1α staining during G1 versus late S-phase.
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MCF7 cells were serum starved (0.05% FCS) for 24 hours before release into medium containing 20% FCS. Cells were X-irradiated (2Gy) at different times and fixed 15 minutes later, such that fixed cell populations were predominantly in G1 or late S-phase. (6.5 hr, 38 hr respectively). Correlation between HP1α and H2AXγ signals was determined by eye from overlaid immunofluorescence images. Mean numbers of nuclei exhibiting the described coincidence of HP1α and H2AXγ signals were derived from at least two fields containing in excess of 100 nuclei.*p
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2015-12-02



