Whole gene expression analysis in offspring liver

Increasing evidence indicates that paternal dietary conditions influence the metabolic disorders of offspring. Previous studies suggested the involvement of epigenetic regulation for such phenomena, but the mechanism remains elusive. To reveal the molecular function of stress-dependent epigenetic regulator ATF7 in paternal inheritance of dietry effect on offpring phenotype, we analyzed ATF7-binding profiles in testicular germ cells by ChIP-seq, DNA methylation profiles in sperm by TGBS, small RNA expression profiles in HRCS by RNA-seq, and whole expression profiles in offspring liver by expression array analysis, using wild-type and ATF7 heterozygous mutant mice fed with control diet or low-protein diet. We performed expression array analysis to identidy genes which expressions are affected by paternal diet and ATF7 mutation. This series contains six sets of exression array data; WT_CD, WT_LPD, ATF7+/–_CD, ATF7+/–_LPD, ATF7+/+_CD and ATF7+/+_LPD. For all data set, we prepared eight CEL files generated by eight biological-independent experiments.