Tele02F-Tele02R project for fish based on environmental DNA technology

In this study, the Chongqing section of the lower reaches of the Fujiang River Basin is divided into six isolated reaches by five DAMS , including Shuangjiang (SS) reservoir area, Tongnan (ST) reservoir area, Fujinba (SF) reservoir area, Anju (SA) reservoir area, Weituo (SW) reservoir area and Weituo (SWX) reservoir area. Three sampling points were set up in each reservoir area, and 18 sampling points were distributed at the head, middle and end of each reservoir area.Water samples were collected during the spawning period (a), feeding period (b) and wintering period (c) from 2023 to 2024. At each sample point, a plexiglass water sampler was used to collect 3L mixed water samples from the head, middle and end of the reservoir, as well as the upper, middle and lower water layers. Then, the water samples from three sampling points in the same sampling section were mixed, and 6L of the mixed water samples were divided into 3 parallel samples on average (every 2L of water sample was used as 1 parallel sample), with a total of 162 water samples, and the remaining water samples were discarded.