The results of sensitivity testing of the real-time PCR array primer sets specific for HIV-1, HIV-2, HBV, HCV, and WNV the with FDA/CBER analytical plasma panels.

1– RNA/DNA, recovered from 1 ml of plasma spiked with a known amount of virus, was converted to cDNA and divided into eight PCR reactions (the dilution factor was equal to 8). Thus, two to three PCR repeats in the same run were performed for each primer set;2- + means the virus was detected in 2 out of 2 or 3 out of 3 PCR repeats for each primer set; four plus (+/+/+/+) means that the virus was detected with all four primer sets. The order of the primers in testing was the following: four primer sets targeting HIV-1: NP3/4, NP51/52, NP170/171 and NP175/174; three primer sets targeting HIV-2: NP84/85, NP86/87 and NP76/77; three primer sets targeting HBV: NP11/97, NP94/100 and NP11/97-mod; two primer sets targeting HCV: NP13/14 and NP13/14-mod; three primer sets targeting WNV: NP 21/22, NP176/177 and NP178/179.