miRNA expression in differentiating primary human bronchial epithelial cells cultured at air-liquid-interface

We performed microarray analysis of miRNA expression in differentiating primary human bronchial epithelial cells. The goal was to identify miRNAs that are dynamically expressed under airway epithelial development. Cells were cultured at air-liquid-interface and were harvested day 4, 6, 8, 11, 13, 15, 18, 20 and 22 for analysis. Cells were cultured at air-liquid-interface and were harvested day 4, 6, 8, 11, 13, 15, 18, 20 and 22 for analysis. Total RNA was labeled with Cy3-CTP and hybridized overnight at 65 deg C in Agilent hybridization buffer. Slides were scanned on an Agilent scanner immediately after washing and scanned images were analyzed with Feature Extraction Software 10.6 (Agilent) using default parameters. Log2 Normalized signal intensity (derived from replicate probes printed on the same array).