Explore plasma exosomal derived lncRNA and mRNA expression in adolescent with major depressive disorder

Major Depressive Disorder (MDD) during adolescence significantly jeopardizes both mental and physical well-being. However, the etiology underlying MDD in adolescents remains unclear. Extracellular vesicles (EVs), long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) played significant roles in regulating various biological processes. 10 drug-naïve adolescents with MDD patients and 10 age, sex matched healthy control were enrolled. Draw whole blood from the cubital vein from participants, plasma was centrifuged at 3500rpm 10min. An exoRNeasy Midi and Maxi Kits were used to isolate the plasma exosome and extract exosomal RNA per the manufacturer’s instruction. Microarray technology was used to detect lncRNAs and mRNAs. Bioinformatics analysis was applied to explore function of differential expression genes and screen candidate genes. 10 drug-naïve adolescent with MDD patients and 10 age, sex matched healthy control were enrolled to isolate plasma from whole blood. Plasma exosomal derived RNA was extracted and microarray technology was used to detect lncRNAs and mRNAs, bioinformatics analysis was applied to explore function of differential expression genes and screen candidate genes.