ImmGen ULI: Deep RNA-seq data

Primary RNAseq data for five highly purified immunocyte populations (splenic NK, Follicular B, naive CD4+ abT, gdT cells and peritoneal macrophages) profiled to unusual depth as multiple biological replicates, using the ImmGen ULI pipeline. RNAseq datasets generated routinely by ImmGen using the ULI (ultra-low-input) pipeline are somewhat shallow, and not suitable for exploration of genes expressed at very low levels. Here, an average of 16 replicates were generated for each of 5 reference cell-types and sequenced using ImmGen's standard RNA-seq pipeline contributor: Immunological Genome Project Consortium本数据集包含5种高度纯化的免疫细胞群的RNA测序（RNAseq）原始数据，涵盖脾脏自然杀伤（NK）细胞、滤泡B细胞、初始CD4+ αβT细胞、γδT细胞及腹腔巨噬细胞，采用ImmGen ULI流程开展多生物学重复的超高深度测序分析。免疫基因组计划（ImmGen）常规使用ULI（超低输入，ultra-low-input）流程生成的RNA测序数据集通常测序深度较浅，不适用于极低表达水平基因的探究。本研究针对5种参考细胞类型各设置了平均16个生物学重复，并采用ImmGen标准RNA测序流程完成测序。 数据集贡献方：免疫基因组计划（Immunological Genome Project）联盟