Effect of LINE1 intervention on vascular calcification (RNA-seq)

In this study, we report the protective effect of LINE1 intervention on vascular calcification. To elucidate the molecular mechanisms underlying the protective role of LINE1 inhibition, we employed RNA sequencing to identify gene expression changes under LINE1 suppression conditions. Our findings provide insights into the pathways involved in modulating vascular calcification and highlight LINE1 as a potential therapeutic target. Overall design: Human vasculr smooth muscle cells exposed to Pi for 7 days and transfected with si-scr or si-LINE1, or treated with NRTIs or not. RNA seq was employed to identify downstream targets of LINE1 inhibition in vascular calcification. Gene expression levels were calculated using the Fragment Per Kilobase of Million mapped reads, and differential expression was assessed using the DEGseq package.