Determination of the cofactor composition of the mAOX3 variants.
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µM molybdenum/µM enzyme) and iron (µM 2 x [2Fe2S]/µM enzyme) contents were determined by ICP-OES (see Experimental procedures).1 Molybdenum ( %.2 Determined after conversion to the stable oxidized fluorescent product FormA. mAOX3-WT was set to 100 Relative molybdopterin (MPT) saturation, molybdenum and iron saturation and ratio of absorbance at 280 nm, 444 nm and 550 nm of mAOX3 variants in comparison to reported values for the wild type enzyme.
创建时间：
2013-12-16
