Expression of HPV 16 E6 in H1299 lung cancer cells.
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HPV 16 E6 expression levels of H1299-HPV16E6 induced by 5 µM ponasterone A (PonA) was significantly higher than that of parental H1299, H1299-pIND and H1299-HPV16E6 treated with 100% alcohol (Alc) or PonA after 24 hours. (A) RNA was isolated and subjected to RT-PCR analysis using specific primers to HPV 16 E6 and GAPDH. (B) Total cell extracts were used to detect HPV 16 E6 and β-actin by Western blot analysis. HPV 16 E6-containing SiHa cervical cancer cells were used as positive control (lane 10).
创建时间：
2016-02-24
