ICEs with regions identical or similar to known single strand origins from plasmids.

aICEs with regions similar to known single strand origins in RCR plasmids were identified using searches with BLASTN [80]. Tn5253 and Tn1311 were identified by BLASTN against the NCBI nucleotide collection database. All other ICEs were identified using WU-BLAST 2.0 against the ICEberg v1.0 ICE nucleotide sequence database [81].bAccession numbers refer to the nucleotide sequence files in GenBank.cThe sso sequences from pC194 (168 nucleotides) and pT181 (234 nucleotides) [29] and pUB110 (340 nucleotides) [82] were used to search for similar sequences in ICEs.ICEs with regions identical or similar to known single strand origins from plasmids.