Hyaluronic acid/PEO electrospun tube reduces tendon adhesion to levels comparable to native tendons – an in vitro and in vivo study

We produced an electrospun DegraPol® tube containing a layer of hyaluronic acid/polyethylene oxide with the aim to use HA as biolubricant and improve the anti-adhesive effect of the DegraPol® tube. As hyaluronic acid/polyethylene oxide was the first time added to DegraPol electrospun scaffolds, material properties were analyzed with SEM, Fourier-transformed infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Water Contact Angle (WCA), and by testing the mechanical qualities. Tubes were applied in a full laceration rabbit Achilles tendon and three weeks after implantation, the adhesion extent of the extracted Achilles tendons was determined using Picrosirius Red staining.

本研究制备了一种含有透明质酸/聚乙二醇层的电纺 DegraPol® 管材，旨在将透明质酸用作生物润滑剂并增强 DegraPol® 管材的防粘附性能。鉴于透明质酸/聚乙二醇层为首次添加至 DegraPol 电纺支架中，因此采用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、差示扫描量热法（DSC）、水接触角（WCA）以及力学性能测试等方法对材料特性进行了分析。管材应用于完全撕裂的兔跟腱，植入三周后，通过使用ピロシリウスレッド染色法确定了提取跟腱的粘附程度。