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Exp_20220430_1_Mucinbeads_longterm_BTRI

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For further investigation of Bacteroides thetaiotaomicron VPI-5482 (DSM 2079T) and Roseburia intestinalis L1-82 (DSM 14610T) growth and their interactions in the long term, an experiment was designed for the co-culture to follow for 288 h with samples taken every 24 h since 120 h after inoculation in Wilkins-Chalgren Anaerobe Broth and mucin beads Notes: live/dead cell staining followed by flow cytometry, using a benchtop CytoFLEX S flow cytometer (Beckman Coulter, Brea, USA) In addition to the instrumental calibration with CytoFLEX Daily QC Fluorospheres, 0.5 μm and 1 μm green-fluorescent beads (Thermo Fisher Scientific, USA) were applied to each batch of experiments as internal standards, to monitor the instrument stability and to make cytometric data from different batches more comparable.
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2024-01-01
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