Deep exome sequencing

Bam files from deep exome sequencing of blood DNA samples from five TET2 mutation carriers (Ly1, Ly2, Ly9, Ly11, Ly14) and three wild-type family members (Ly8, Ly10, Ly13) extracted at multiple time points. Library preparations were performed with SeqCap EZ Exome v3 (Roche, Switzerland) using six different index primers per sample for which paired-end Illumina sequencing was done with 75bp read length and HiSeq4000 sequencer. After alignment (bwa version 0.7.12), base recalibration (GATK 3.5), realignment around indels (GATK 3.5) and duplicate removal (MarkDuplicates; Picard Tools version 1.79), data from libraries with six different indexes were merged.

本数据集包含来自五位TET2突变携带者（Ly1、Ly2、Ly9、Ly11、Ly14）和三位野生型家族成员（Ly8、Ly10、Ly13）的多时间点血液DNA样本的深度外显子测序Bam文件。通过使用Roche公司（瑞士）的SeqCap EZ Exome v3进行文库制备，每个样本采用六种不同的索引引物，并使用Illumina测序平台进行双端测序，读长为75碱基，使用HiSeq4000测序仪。在bwa版本0.7.12进行比对后，采用GATK 3.5进行基线校正、围绕插入/缺失的重新比对（GATK 3.5）以及重复去除（MarkDuplicates；Picard Tools版本1.79），将具有六种不同索引的文库数据进行了合并。