Acquisition of optimal human follicular helper CD4 T cell function is defined by specific molecular, positional and TCR dynamic signature

The purpose of this study was to study TFH cells and their transitionaing Status and its association with positioning in the Germinal Center. We observed significant transcriptomic signatures associated with CD57hi TFH status. To use sorted tonsillar CD4 subsets for analysis of transcriptome signatures and investigate whether these signatures are associated with other biological factors of these CD4 T cell subsets. Cells were collected and lysed for RNA extraction. Reverse transcription reactions were performed to obtain cDNAs which were hybridized to the Illumina Human HT-12 version 4 Expression BeadChip according to the manufacturer’s instruction, and quantified using an Illumina iScan System. The data were collected with Illumina GenomeStudio software.