Single-cell Sequencing Analysis Identifies Intratumoral Immune Cell Population in Head and Neck Squamous Cell Carcinoma from 4NQO-induced Mice upon JQ1 treatment

In this study, we aimed to identify the landscape alterations of the subpopulations of the tumor-infiltrating immune cells in HNSCC from 4NQO-induced mice upon JQ1 treatment; by using single cell sequencing analysis, we characterized the alterations of intratumoral T cell subpopulations by separating T cells and yielded 9 distinct T cell subpopulations defined by the distribution of classical marker genes, including Tregs, naïve-like cell, γδ T cells 1, IFNG+ HSP+ T cell, CD8+ cytotoxic T cell, CD4+ T effector cell, γδ T cells 2, Xcl1+ T cells and ILC2. In comparison with mice treated with control, JQ1 treatment resulted in an increase of the percentage of CD8+ T cells and Xcl1+ T cells, indicating an activated tumor immune microenvironment in primary HNSCC upon JQ1 treatment. Single-cell transcriptomic analysis of murine HNSCC from 4NQO-induced mice treated with JQ1 and control.