RNA-dan_vs_mod.result.xls

The sequencing was conducted by Shanghai Zhongke New Life Biotechnology Co., Ltd. mRNA from eukaryotic organisms was enriched using magnetic beads with Oligo (dT). Subsequently mRNA was fragmented using a fragmentation buffer. Using mRNA as a template, the first strand of cDNA was synthesized using random hexamers, followed by the addition of buffer, dNTPs, and DNA polymerase I to synthesize the second strand of cDNA. Subsequently, double-stranded cDNA was purified using AMPure XP beads. The purified double-stranded cDNA underwent end repair A-tailing, and ligation of sequencing adapters, followed by size selection using AMPure XP beads and finally PCR enrichment to obtain the ultimate cDNA l<br>