Identification and characterization of pluripotency associated lncRNAs in human iPS cells

Induced pluripotent stem cells (iPSCs) are an attractive source of stem cells for many applications like patient-specific cell therapy, disease modeling or drug screening. Generation of iPSCs by cellular reprogramming is a complex and highly inefficient process that involves a high number of genetic and epigenetic modifications most of them unknown. Those genetic modifications include both coding and non-coding genes like long non-coding RNAs (lncRNAs). A detailed knowledge of the factors that make a cell to return to its own origin would help to a deeper understanding of the reprogramming process and to address new strategies that solve the current problems within this field. Total RNA was extracted from five human iPSCs generated form adipose derived stem cells (ADSCs) and three parental ADSCs. Then RNA was hybridized to SurePrint G3 Human GE Microarray, Agilent Technologies.