Mapping of p300/CBP binding sites in MDA-MB-231 cells transfected with a control siRNA or a siRNA directed against Fra-1(FOSL1)

In the article "Fra-1 regulates its target genes via binding to remote enhancers without exerting major control on chromatin architecture in triple negative breast cancers" by Bejjani et al., we mapped p300/CBP binding sites in MDA-MB-231 cells transfected with a control siRNA or a siRNA directed against Fra-1(FOSL1) to study whether Fra-1 can modulate p300/CBP recruitment on MDA-MB-231 genome MDA-MB-231 cells were transfected with a control siRNA or a siRNA directed against Fra-1. ChIP-seq were carried out using an mouse monoclonal antibody specific of p300/CBP (sc-32244X from Santa Cruz Biotechnology)