Time Dependent Analysis of Rat Microglial Markers in Traumatic Brain Injury Reveals Dynamics of Distinct Cell Subpopulations

The repository contains seven zip files for flow cytometry data for rat microglia following Controlled cortical impact (CCI) collected at seven time points: 3 hours, 1 day, 2 days, 7 days 14 days, 21 days and 28 days.

Each file contains 24 CSV files converted from the raw fcs file and text files that contain meta data about the file.

Each file name depicts the information of whether it is Panel A (M1 in the file name) or Panel B (M2 in the file name) measurement and whether the measurement was conducted on the ipsilateral (ipsi) or contralateral (contra) side of the CCI, or sham conducted on the ipsilateral (ipsi) or contralateral (contra) sides.

Below is the description of the columns of each file:

File column name

Original name

Panel A (M1) context

PAnel B (M2) context

Description

F0

FSC-A

FSC-A

FSC-A

Forward scattering Area

F1

FSC-H

FSC-H

FSC-H

Forward scattering Height

F2

FSC-W

FSC-W

FSC-W

Forward scattering Width

F3

SSC-A

SSC-A

SSC-A

Side scattering area

F4

FITC-A

FITC-A

FITC-A

Fluorescein isothiocyanate

F5

PE-A

CD32

CD200R

Protein expression

F6

PerCP-Cy5-5-A

-

CD163

Protein expression

F7

PE-Cy7-A

CD11

CD11

Protein expression

F8

BV421-A

P2Y12

P2Y12

Protein expression

F9

BV510-A

-

-

Dye 

F10

Alexa Fluor 647-A

CD86

RT1B

Protein expression

F11

APC-Cy7-A

CD45

CD45

Protein expression

F12

Event Time

Event Time

Event Time

Event Time