Rosalind Silverman-Gavrila (2011) CIL:8102, Drosophila melanogaster. CIL. Dataset

This movie is of a Drosophila melanogaster early embryo (cycle 12) expressing GFP-tubulin (green) and injected with Rhodamine-labeled histones (red) and 8mg/ml RanT24N, a dominant negative inhibitor of spindle assembly). In this movie, the relative organization of microtubules (green) and chromosomes (red) can be seen. In early stages, Drosophila embryos divide syncytially. When spindles assemble, chromosomes attach to kinetochore microtubules and then they congress to the metaphase plate and disjoin in anaphase. Next they move to the poles and when they reach the poles in telophase they decondense and two daughter nuclei form. A low dose of RanT24N, a dominant negative mutant of Ran, causes mild defects in spindle assembly and chomosome division. In the middle of the field of view, the assembly of some spindles is inhibited and some form as a circle of microtubules around the chromosomes. At the edge of the field of view, spindles assemble but some microtubules grow away from the spindle. In some cases, nuclei do not divide. In others, chromosomes do not align properly, or lag behind the rest in anaphase. And, in some cases, permanent or resolving bridges occur between the daughter nuclei.

Time lapse 4D spinning disk confocal microscopy images were acquired every 8 seconds and are played at 6 frames/second. The images were acquired with a Nikon microscope (a X40 objective) and a Perkin-Elmer laser spinning disk. The program used to capture, pseudocolor, and process the images was Metamorph.

本数据集记录了黑腹果蝇（Drosophila melanogaster）早期胚胎（12周期）的表达GFP-微管蛋白（绿色）和注射有罗丹明标记的组蛋白（红色）及8mg/ml RanT24N（一种纺锤体组装的显性负抑制因子）的动态图像。在此图像中，可以观察到微管（绿色）和染色体（红色）的相对组织结构。在早期阶段，黑腹果蝇胚胎进行合胞体分裂。当纺锤体组装时，染色体附着于着丝粒微管上，随后它们汇聚至中期板并在线粒期分离。随后，它们移动至两极，在终变期到达两极时解聚，从而形成两个子核。RanT24N（Ran的显性负突变体）的低剂量导致纺锤体组装和染色体分离的轻微缺陷。在视野中央，某些纺锤体的组装受到抑制，并围绕染色体形成微管环。在视野边缘，纺锤体组装，但部分微管从纺锤体生长出来。在某些情况下，细胞核未进行分裂。在其他情况下，染色体未能正确对齐，或在后期滞留于其他染色体之后。此外，在某些情况下，子核之间出现永久性或可解消的桥。时间分辨4D转盘共聚焦显微镜图像每隔8秒采集一次，并以每秒6帧的速度播放。图像采集使用尼康显微镜（X40物镜）和珀金埃尔默激光转盘，图像的采集、伪彩色处理及后期处理均使用Metamorph软件完成。