Single-cell RNA-sequencing analysis reveals the peripheral immune characteristics of patients with primary membranous nephropathy

Primary Membranous Nephropathy (PMN) is characterized by dysregulated immune responses, with B and T cells playing critical roles in disease pathogenesis. While significant strides have been made in identifying specific autoantigens and leveraging monoclonal antibodies, the precise immunopathogenic mechanisms underlying immune cell involvement in PMN remain elusive. To comprehensively characterize the cellular, molecular, and immunological landscape, we employed single-cell RNA sequencing (scRNA-seq) on peripheral blood mononuclear cell samples (PBMC) obtained from 6 patients with PMN and 3 healthy controls (NC).We examined the heterogeneity and functional diversity of B cells in PMN. These results offer new insight into pathogenesis and identify new therapeutic targets for PMN. Single-cell RNA sequencing (scRNA-seq) was applied to PBMC from 6 MN and 3 control subjects to define the transcriptomic landscape at the single-cell resolution.