Data from: Development of a 3D simulator for training the mouse in utero electroporation

In utero electroporation (IUE) requires high-level training in
microinjection through the mouse uterine wall into the lateral ventricle
of the mouse brain. Training for IUE is currently being performed in live
mice, as no artificial models allow simulations yet. This study
aimed to develop an anatomically realistic 3D-printed simulator to train
IUE in mice.  To this end, we created embryo models containing
lateral ventricles. We coupled them to uterus models in six steps: (1)
computed tomography imaging, (2) 3D model segmentation, (3) 3D model
refinement, (4) mold creation to cast the actual model, (5) 3D mold
printing, and (6) mold casting the molds with a mix of soft silicones to
ensure the hardness and consistency of the uterus and embryo. The results
showed that the simulator assembly successfully recreated the IUE. The
compression test did not differ in the mechanical properties of the real
embryo or in the required load for uterus displacement. Furthermore, more
than 90% of the users approved the simulator as an introduction to IUE and
considered that the simulator could help reduce the number of animals for
training. Despite current limitations, our 3D simulator enabled a
realistic experience for initial approximations to the IUE and is a real
alternative for implementing the 3Rs. We are currently working on refining
the model.