RNAseq of Brassica napus seed

mRNA expression profiling of the embryo, endosperm (micropylar, peripheral, chalazal), and seed coat (outer, inner, chalazal, chalazal proliferating tissue) of the developing Brassica napus seed. Tissues were isolated using laser microdissection (LMD) from Brassica napus seeds at the globular, heart, and mature green stages of seed development. To investigate the transcriptome of the B. napus seed throughout space and time, we sampled seeds across four developmental stages (ovule, globular, heart, and mature green) We fixed seeds from each stage and embedded them in parrafin wax, then dissected out four fililal subregions (embryo proper, micropylar endosperm, peripheral endosperm, chalazal endosperm) and four maternal subregions (inner seed coat, outer seed coat, chalazal seed coat, and chalazal proliferating tissue) using laser microdissection (LMD) We then performed RNA-seq on the samples from each seed stage and each available subregion.