DataSheet_1_Prussian Blue Scavenger Ameliorates Hepatic Ischemia-Reperfusion Injury by Inhibiting Inflammation and Reducing Oxidative Stress.pdf

Oxidative stress and excessive inflammatory responses are the two critical mechanisms of hepatic ischemia-reperfusion injury (HIRI) encountered in many clinical settings, including following hepatectomy and liver transplantation. Effective anti-inflammatory and anti-oxidative pharmacological interventions are urgently needed to counter HIRI. The present study showed that a biocompatible Prussian blue (PB) scavenger with reactive oxygen species (ROS) scavenging and anti-inflammatory properties might be used a promising treatment for HIRI. Following intravenous administration, PB scavenger was mainly distributed in the liver, where it showed excellent ability to alleviate apoptosis, tissue injury and organ dysfunction after HIRI. PB scavenger was found to protect liver tissue by scavenging ROS, reducing neutrophil infiltration and promoting macrophage M2 polarization. In addition, PB scavenger significantly reduced oxidative stress in primary hepatocytes, restoring cell viability under oxidative stress condition. PB scavenger effectively reduced lipopolysaccharide-stimulated inflammation in RAW 264.7 cells. These findings indicate that PB scavenger may be a potential therapeutic agent for the treatment of HIRI, providing an alternative treatment for ROS-associated and inflammatory liver diseases.

氧化应激与过度炎症反应是众多临床场景中，包括肝切除术及肝移植术后所遭遇的肝缺血再灌注损伤（HIRI）的两种关键机制。针对HIRI，迫切需要有效的抗炎和抗氧化药理学干预措施。本研究表明，一种具有活性氧清除（ROS）和抗炎特性的生物相容性普鲁士蓝（PB）清除剂可能成为治疗HIRI的颇具潜力的治疗方法。经静脉给药后，PB清除剂主要分布在肝脏，表现出卓越的缓解HIRI后细胞凋亡、组织损伤和器官功能障碍的能力。研究发现，PB清除剂通过清除ROS、减少中性粒细胞浸润并促进巨噬细胞M2极化，保护肝脏组织。此外，PB清除剂显著降低了原代肝细胞中的氧化应激，在氧化应激条件下恢复了细胞活力。PB清除剂有效减少了RAW 264.7细胞受脂多糖刺激引发的炎症。这些发现表明，PB清除剂可能成为治疗HIRI的潜在治疗药物，为ROS相关性和炎症性肝脏疾病的治疗提供了一种替代疗法。