Regulation of Xylem transcriptome by water supply and potassium nutrition

(1) Wood production in fast-growing Eucalyptus grandis trees is highly dependent on both potassium (K) fertilization and water availability but the molecular processes underlying wood formation in response to the combined effect of these two limiting factors are still unknown. E. grandis trees were submitted to four combinations of K fertilization and water supply. Transcriptomic analyses (RNAseq) were performed to study the crosstalk responses triggered by water availability and potassium supply at the level of gene expression. Three sequencing libraries per treatment were generated and sequenced (Illumina, 150 bp paired-end reads) by the Genotoul GeT platform (https://get.genotoul.fr/en/; France) using the HiSeq3000 (Illumina). An average of 46 million reads per library was generated, >90% were mapped to E. grandis reference genome (v1.1, https://phytozome.jgi.doe.gov/) using TopHat2 (Kim et al., 2013).