MicroED datasets of biotin collected on Titan Krios G4 operated at 300kV and Ceta-D camera

MicroED datasets of biotin microcrystals were collected using Titan Krios G4 (300 kV) with the CMOS camera Ceta-D. The stage was controlled using SerialEM and diffraction images were independently collected using Velox software. For this reason, only frames with constant rotation speed should be used for data processing.

Rotation step (continuous) was ~0.96°/frame and each dataset consisted of ~63 images (tilt range is +/-30°). The calibrated camera lengths using evaporated aluminum were 751.09 mm, 952.85 mm, and 1075.09 mm, corresponding to nominal lengths of 430 mm, 540 mm, and 610 mm, respectively. Biotin crystals belonged to space group P212121 with a~5.2, b~10.2, c~20.8 Å, and could be merged at ~0.6 Å resolution.

Collection conditions:

gun lens 5, spot 11, C2 aperture 20, beam size 1.5 μm, 0.033 e/Å2/sec

Note:

emd files (hdf5 format) were transparently compressed using h5repack -f SHUF -f GZIP=4 command to reduce file size.

EMD file can be processed with DIALS using this dxtbx format file.

Metadata (machine parameters, stage tilt angles etc.) is stored as json format in /Data/Image/*/Metadata in emd file. See here for details.

If you want to process data using DIALS, please see the processing note.