SNAT - Single cell RNA sequencing of mouse sorted Schwann cells at postnatal day 1 (P1)

Single cell RNA sequencing of sorted mouse Schwann cells expressing eYFP due to P0Cre-mediated recombination. Schwann cells were acutely extracted from P1 mouse pups which, besides of beta-actin dsred, also express eYFP upon P0cre-mediated recombination. The eYFP fluorescence allows the selection of eYFP positive Schwann cells from eYFP negative debris and non-Schwann cells in the nerve. 384-well plates were used to collect one cell per well, and these individual Schwann cells were further processed for library preparation using the SmartSeq2 chemistry. Two of these plates were sequenced at P1, each plate with cells derived from independent biological samples. The cells sorted into each plate derive from an original cell suspension containing pooled cells extracted from male and female mice in a 1:1 ratio.本数据集为基于P0Cre介导重组表达eYFP的分选小鼠雪旺细胞（Schwann cells）的单细胞RNA测序数据。实验所用雪旺细胞新鲜分离自出生后1天（P1）的幼鼠，该品系小鼠除表达β-肌动蛋白-红色荧光蛋白（β-actin dsRed）外，经P0Cre介导重组后可表达eYFP。借助eYFP荧光信号，可从神经组织的eYFP阴性碎屑及非雪旺细胞中分选得到eYFP阳性雪旺细胞。实验采用384孔板实现单孔单细胞捕获，随后利用SmartSeq2建库体系对单个雪旺细胞完成文库制备。其中两块384孔板的细胞在P1阶段进行测序，每块板的细胞均来自独立的生物学重复样本。分入各孔板的细胞均来自混合细胞悬液，该悬液由雌雄比例为1:1的小鼠来源细胞混合制备而成。