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Primers and probe sequences, estimated annealing temperatures (Tm), and optimal primer concentrations of the eDNA assay for bull trout ITSI.

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Figshare2018-11-06 更新2026-04-29 收录
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https://figshare.com/articles/dataset/Primers_and_probe_sequences_estimated_annealing_temperatures_Tm_and_optimal_primer_concentrations_of_the_eDNA_assay_for_bull_trout_ITSI_/7303286
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Optimal primer concentrations refer to the lowest concentration of primers resulting in the earliest Ct while maintaining a high end-point fluorescence. The assay amplifies a 172-nucleotide fragment of the bull trout ITSI gene.
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2018-11-06
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