Primers and probe sequences, estimated annealing temperatures (Tm), and optimal primer concentrations of the eDNA assay for bull trout ITSI.
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Optimal primer concentrations refer to the lowest concentration of primers resulting in the earliest Ct while maintaining a high end-point fluorescence. The assay amplifies a 172-nucleotide fragment of the bull trout ITSI gene.
创建时间：
2018-11-06
