Data_Sheet_1_Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle.PDF

Tissue engineered skeletal muscle allows investigation of the cellular and molecular mechanisms that regulate skeletal muscle pathology. The fabricated model must resemble characteristics of in vivo tissue and incorporate cost-effective and high content primary human tissue. Current models are limited by low throughput due to the complexities associated with recruiting tissue donors, donor specific variations, as well as cellular senescence associated with passaging. This research presents a method using fused deposition modeling (FDM) and laser sintering (LS) 3D printing to generate reproducible and scalable tissue engineered primary human muscle, possessing aligned mature myotubes reminiscent of in vivo tissue. Many existing models are bespoke causing variability when translated between laboratories. To this end, a scalable model has been developed (25–500 μL construct volumes) allowing fabrication of mature primary human skeletal muscle. This research provides a strategy to overcome limited biopsy cell numbers, enabling high throughput screening of functional human tissue.

组织工程化骨骼肌模型允许探究调节骨骼肌病理学的细胞和分子机制。该制造模型必须与体内组织的特征相似，并融入高效且高含量的初级人源组织。当前模型由于招募组织供体、供体特异性变异以及传代过程中与细胞衰老相关的复杂性，导致通量较低。本研究提出了一种使用熔融沉积建模（FDM）和激光烧结（LS）3D打印的方法，以生成可重复和可扩展的组织工程化初级人源肌肉，其中具有与体内组织相似的排列成熟的肌管。许多现有模型因定制化而造成实验室间转换时的可变性。为此，开发了一种可扩展模型（构建体积为25-500 μL），允许制造成熟的初级人源骨骼肌。本研究提供了一种策略，以克服有限活检细胞数量的问题，从而实现功能性人源组织的快速筛选。