Transcriptome_of_the_CAST_MOE

This study aims to probe the transcriptome of the main olfactory epithelium (MOE) of castaneus mice. At 12 weeks, MOEs were dissected from three biological replicates. Additionally, to assess the specificity of RNAseq when studying olfactory receptors (ORs), we dissected the MOE of three biological replicates of a mutant strain of mice that has a deletion comprising over a hundred OR genes in the Olfr7 cluster in chromosome 9. RNA was extracted using the Qiagen RNeasy kit using standard protocols, with on-column DNase digestion. Each three RNA samples were multiplexed together and sequenced in one lane on the Illumina HiSeq platform.