NMR Metabolomic response to exercise in young and older human plasma

Background: The favorable health-promoting adaptations to exercise result from cumulative responses to individual bouts of physical activity. Older adults often exhibit anabolic resistance; a phenomenon whereby the anabolic responses to exercise and nutrition are attenuated in skeletal muscle. The mechanisms contributing to age-related anabolic resistance are emerging, but our understanding of how chronological age influences responsiveness to exercise is incomplete.
The objective was to determine the effects of healthy aging on peripheral blood metabolomic response to a single bout of resistance exercise and whether any metabolites in circulation are predictive of anabolic response in skeletal muscle.
Methods: Thirty young (20-35 years) and 49 older (65-85 years) men and women were studied in a cross-sectional manner.  Participants completed a single bout of resistance exercise consisting of eight sets of 10 repetitions of unilateral knee extension at 70% of one-repetition maximum. Blo..., , , # NMR Data Files

[https://doi.org/10.5061/dryad.2547d7x00](https://doi.org/10.5061/dryad.2547d7x00)

Nuclear Magnetic Resonance (NMR) spectra were obtained from plasma samples from young and older individuals at rest and at timepoints (immediate post, 30 minutes, 90 minutes, and 180 minutes) following a single bout of exercise. Proton spectra were acquired using a Bruker 600 MHz Advance III HD spectrometer. Spectra were analyzed using the remote automated Bruker Data Analysis server, which annotates known metabolites based on chemical shifts. The individual PDF files represent de-identified NMR metabolomics reports for each sample that include quantitative data on each analyze as well as the original spectrum for each analyze, signal fitting, and reference ranges. The accompanying excel file provides information on age group, sex, and timepoint for each file.

## Description of the data and file structure

The proton NMR spectra for each plasma sample were analyzed using the Bruker ...