Whole genome sequencing of Histoplasma capsulatum SG1 yeast-locked mutant strain

Dimorphic fungi are temperature-sensitive organisms that couple their cell shape with their environment. One of these fungi, Histoplasma capsulatum, exists as both a soil-dwelling hypha and a host-associated yeast. We performed a genetic screen based on insertion of a mobile element (T-DNA) to identify mutants that are unable to transition from the yeast form to the hyphal form. Here, we performed paired-end deep sequencing of the SG1 yeast-locked mutant strain in order to determine the site and nature of the T-DNA insertion.